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Gene Expression - Microarrays Laboratory  web page

 

It is not possible to research on a large number of genes using traditional methods. Microarrays techology enables the researchers to investigate and address issues which were once thought to be non traceable. With microarrays technology one can analyze the expression of many genes in a single reaction quickly and in an efficient manner. Microarrays technology has empowered the scientific community to understand the fundamental aspects underlining the growth and development of life as well as to explore the genetic causes of anomalies occurring in the functioning of the human body.

Microarray Technique

A typical microarray experiment involves the hybridization of an mRNA molecule to the the DNA template from which it is originated. An array is an orderly arrangement of samples where matching of known and unknown DNA samples is done based on base pairing rules. An array experiment makes use of common assay systems such as microplates or standard blotting membranes. The sample spot sizes are typically less than 200 microns in diameter usually contain thousands of spots. Thousands of spotted samples known as probes (with known identity) are immobilized on a solid support (a microscope glass slides or silicon chips or nylon membrane). The spots can be DNA, cDNA, or oligonucleotides. These are used to determine complementary binding of the unknown sequences thus allowing parallel analysis for gene expression and gene discovery. An experiment with a single DNA chip can provide information on thousands of genes simultaneously.

  • gene expression and microRNA: profiling and data analysis
  • bioinformatics: statistics and information retrieval

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